Flagella Staining by Loeffler’s Method and its Details

Flagella Staining by Loeffler’s Method and its Details

Flagella is the most important locomotory organ of a bacterial cell and for studying this structure it is important to stain the flagella.So here we have another staining technique used for staining of flagella that is Loffler’s staining technique.

There are some other staining techniques that are also used for staining of flagella that are Bailey’s method,Gray’s method, Casares-Gil’s method and Leifson’s method.

Here in this article we are going to study  Loffler’s method in detail.


To stain flagella of a cell by using Loffler’s method.


  1. Flagellated cell culture.
  2. Loeffler’s mordant.
  3. Loeffler’s stain.
  4. Distill water.
  5. Chromic acid.


  1. The slides are cleaned by boiling them in chromic acid solution.
  2. The smear is prepared as given in the flow chart below.
  3. Further after smear preparation the slide is treated with Loeffler’s mordant and heated it till steam appears approximately it takes 3 minutes.
  4. After that slide is gently washed by distill water.
  5. Further slide is treated with Loeffler’s stain and heated till steam appears.
  6. Finally slide is washed with water,air dried and observe under oil immersion.

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Stain and Staining Procedures

Stain and staining procedures


Micro-organism can be identified by microscopic examination.These micro-organisms are very small, transparent, and invisible to our naked eyes so it is very difficult to observe these micro-organisms.

These microbial cells are colourless so when these cells are suspended in the aqueous medium the refractive index of the cell and aqueous medium is very low and same and due to lack of contrast observation of such unstained organism is very difficult. So staining of micro-organism in microbiology is an essential step for bacteriological studies.

Purpose of staining

  • Staining procedure helps in the production of contrast between micro-organism an aqueous medium.
  • It is useful to study morphological of bacteria.
  • Staining helps in studying internal as well as external characters of bacteria.


1. Dye – Dye is an organic compound made up of auxochrome and chromophore group these groups are linked to a benzene ring. Dye is used for colouring non-biological objects.

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