Structure of Typical Bacterial Cell

Structure of Bacterial Cell Bacterial cell The bacterial cell is made up of various internal as well as external anatomical structure. Introduction A bacterial cell shows various parts and these parts have specific structure and functions. Some structures are present in that particular species and hence that structure is characteristic feature of that species. Various … Read moreStructure of Typical Bacterial Cell

Flagella Staining by Loeffler’s Method and its Details

Flagella Staining by Loeffler’s Method and its Details

Flagella is the most important locomotory organ of a bacterial cell and for studying this structure it is important to stain the flagella.So here we have another staining technique used for staining of flagella that is Loffler’s staining technique.

There are some other staining techniques that are also used for staining of flagella that are Bailey’s method,Gray’s method, Casares-Gil’s method and Leifson’s method.

Here in this article we are going to study  Loffler’s method in detail.

Aim

To stain flagella of a cell by using Loffler’s method.

Requirement

  1. Flagellated cell culture.
  2. Loeffler’s mordant.
  3. Loeffler’s stain.
  4. Distill water.
  5. Chromic acid.

Procedure

  1. The slides are cleaned by boiling them in chromic acid solution.
  2. The smear is prepared as given in the flow chart below.
  3. Further after smear preparation the slide is treated with Loeffler’s mordant and heated it till steam appears approximately it takes 3 minutes.
  4. After that slide is gently washed by distill water.
  5. Further slide is treated with Loeffler’s stain and heated till steam appears.
  6. Finally slide is washed with water,air dried and observe under oil immersion.

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Flagella Staining Technique by Liefson’s Method

Flagella Staining Technique by Liefson’s Method

Introduction

  • Bacteria have two types of locomotory organs and that are Flagella and pili.
  • Here we are studying about flagella staining.
  • Flagella is a thin,hair like structure made up protein called as flagellin.
  • It size ranges from 20 µ to 200 µ in length.
  • Flagella is one of the most important locomotory organ .It is mainly made up of three parts- 1) Basal body 2) Filament 3) Hook.
  • Flagella is generally present in rod shape bacteria and very few cocci shape bacteria posses flagella.
  • As flagella are very thin and hair like they cannot be easily observed under microscope.
  • So a special technique is design to increase thickness of flagella as well as stain it.
  • Due to these technique we can observe structure of flagella easily under microscope.

Please check out the below video for Flagella staining by Liefson’s method.

Aim

To Stain Flagella of cell by using Leifson’s method

Requirement

  1. Flagellated cell culture slant.
  2. Leifson’s stain.
  3. 1 % Methylene blue.
  4. Distilled water.

Procedure

  • First of all take two hours old flagellated cell culture slant and add two to three drops of sterile distill water in the slant with the help of sterile pipette.
  • Note that the distill water is added slowly without disturbing the growth of cells.
  • After addition of distill water incubated the slant for 20 minutes.
  • Then take a drop of suspension from the slant and place the drop on a clean slide which is kept in slanting position.
  • The drop should flow slowly from one end of slide to other end to avoid folding of flagella on cell.
  • Allow smear to air dry here we don’t use heat fixation treatment .
  • After air drying the slide is flooded with Leifson’s stain till a thin film of shinny surface appear.
  • After this give a gentle stream of water wash treatment to a slide.
  • Now treat the slide with 1 % methylene blue treatment for 1 minute.
  • Give the slide water wash treatment ,air dry and observe under oil immersion lens.

Read moreFlagella Staining Technique by Liefson’s Method

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