Gram Staining Procedure and its Details
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- Gram staining procedure was discovered by Han’s Christian Gram in 1884.
- Gram staining is a universal staining technique used for identification and classification of organisms.
- In this staining, method bacteria are classified into two groups that are-
1. Gram-positive bacteria
2. Gram-negative bacteria
- This classification of bacteria depends upon the property of a cell to retain or lose the primary stain after the treatment of decolourising agent.
- Gram staining is a basic and widely used technique.
- This technique was modified by many scientists but the best result was obtained by Hucker and Conn’s modification.
- A clean grease free slide.
- Bacterial cell suspension.
- Nichrome Wire loop.
- Primary stain – Crystal violet.
- Mordant- Gram’s Iodine.
- Decolourising agent- 95% alcohol ( 95% Ethanol).
- Counterstain- Basic fuschin or Safranin.