Endospore staining by Bartholomew and Mittwer’s Method Aim To stain Endospore of a bacterial cell by Bartholomew and Mittwer’s Method. Requirement Cell suspension of endospore producing bacteria. Malachite green stain. Saffranin stain. Procedure Take a clean grease free slide and prepare a thick smear on a slide. The smear is heat fixed by passing the slide… Read More


Endospore Staining by Dorner’s Method Introduction Endospore is a resistant refractile structure formed inside the cell. Endospore is generally formed by some gram positive bacteria. When a bacteria come across some unfavorable conditions it gives rise to resistant structure called as endospore. These endospore is metabolically inactive,it has low moisture content and it is made… Read More


Volutin Granule Staining Technique and its Details Introduction Micro-organism have a special characteristic these organism store reserve food material in the form of granules.These granules are utilized by these organism when they are in starvation mode. These reserve food granules are also called Volutin granules, Metachromatic granules or Babes Ernst granules. These granules are mainly… Read More


Capsule Staining Technique by Maneval’s Method. Maneval’s staining method is a negative staining method. Aim To stain a  capsule of a cell by Maneval’s staining method. Requirement 1 % Congo red solution. Maneval’s stain. Capsulated cell culture. Please watch the Video given Below Procedure Take a loopful of capsulated cell suspension on a clean grease free… Read More


Capsule Staining Technique by Hiss method Introduction Capsule is the rigid, slimy and gummy covering of the bacterial cell which lies external to the cell wall. The cell that posses the capsule are called as capsulated cell and those who lack the capsule are called as the non-capsulated bacteria. Capsule is mainly made up of… Read More


Cell wall staining by Chances method Please watch the video given below Introduction Cell wall staining technique is a special staining technique in which we especially stain the cell wall of the bacterial cell. As we know cell wall is the outer most rigid covering of cell and depending upon the structure of cell wall… Read More


Acid Fast Staining Technique and its Details Introduction In nature, there is a variety of micro-organism each micro-organism have some special characters. Most of the microorganisms are easily stained by simple staining procedures. But there is some micro-organism that is not easily stained by this technique because they have a waxy covering on its surface.… Read More


Gram Staining Procedure and its Details Please watch the video given below Introduction Gram staining procedure was discovered by Han’s Christian Gram in 1884. Gram staining is a universal staining technique used for identification and classification of organisms. In this staining, method bacteria are classified into two groups that are-          … Read More


Negative Staining Method and its Mechanism Introduction Negative staining procedure is also called as Relief staining. Some bacteria have non-ionic charge or slime layer on their surface. Because of which the stain doesn’t penetrate inside the cell and the bacterial cells are not easily stained. In case of such bacterial cells negative method is used for… Read More


Simple Staining Procedure and its Mechanism Introduction Simple staining is a method of staining in which bacteria are stained by using a single stain. Simple staining is also called as monochrome staining or positive staining. Examples of simple stain are Methylene blue, Safranin, Malachite green, Basic fuchsin and crystal violet etc. In simple staining procedure… Read More