The growth of a Bacterial Cell It can be defined as an orderly increase in the quantity of all cell components of a cell.The cell increases in its size, shape and weight and further undergoes cell division.The bacterial cell undergoes different growth phases.This growth phases of the bacterial cell can be studied by plotting a… Read More

Nitrate Reduction Test Nitrate is a poly-atomic inorganic molecule and its molecular formula is NO3–   Nitrate is reduced by micro-organism in two ways. 1. Assimilatory Nitrate reduction. Assimilatory nitrate reduction mainly occurs during the anaerobic condition in absence of nitrogen. In this reduction process, nitrate and ammonia are used for the synthesis of organic… Read More

Catalase Biochemical Test Introduction Catalase is the name of an enzyme. This enzyme is nearly present in all living plants and animals which are always exposed to oxygen. Catalase enzyme is also present in aerobic and facultative anaerobic micro-organisms. Generally, this micro-organisms utilise oxygen and produce hydrogen peroxide and this hydrogen peroxide is toxic to… Read More

How is the IMViC Biochemical test performed? In the IMViC test there are four biochemical tests and this test is carried out individually. IMViC test is carried out to identify members of the Enterobacteriaceae family. Each letter of IMViC test stand for individual test I – Indole production test. M – Methyl red test. V-… Read More

Carbohydrate Fermentation Test Whenever a new micro-organism is discovered it becomes necessary to identify and catalogue it. For identification of this new microbe, it is first isolated and microscopically examined, and then further biochemically tested. Once these steps are carried out, we can identify this micro-organism. Biochemical tests are essential for the identification and classification… Read More

Disinfection process Can a simple disinfection process avoid infection and diseases? Disinfection process is one of the methods which is used to kill micro-organism. In this process, a chemical substance called as a disinfectant is applied on the surface of living and non-living objects to kill micro-flora. Disinfection process kills almost all micro-organisms except some resistant fungal… Read More

Super Bug Bacteria | A Global Threat A Super bug can be defined as bacteria which are resistant to multiple drugs or antibiotics. These super bugs have become a global threat. Regular disease-causing bacteria have been getting tougher to kill and could get converted into a super bug. Such a super bug would remain unaffected… Read More

Microbial Culture Media Composition Part-3 Here are some of the important culture media composition.   Role a) Eosin Methylene blue broth is used for isolation of coliforms. b) Eosin and methylene blue are aniline dye used to inhibit Gram positive and fastidious gram negative bacteria. Role Blood agar is used for isolation of cultivation and isolation… Read More

Microbial Culture Media Composition Part-2 Please Watch the video given below Continued from Microbial Culture Media Composition Part-1 Some important media composition are given below. Role It is used in oxidative – fermentative test . a) Peptone is a source of carbon,nitrogen and energy. b) NaCl maintain osmotic balance. c) K2HPO4   acts as a buffer. d)… Read More

Microbial Culture Media Composition Part-1 In nature, their are a variety of micro-organisms present and every single organism has some specific growth requirements like suitable temperature, pH, salt concentration nutrient requirements etc. If we want a desired culture of micro-organism we should provide them with the optimum and required growth conditions. On the basis of… Read More