Nitrate Reduction Test

Nitrate Reduction Test Nitrate is a polyatomic inorganic molecule and its molecular formula is NO3–   Nitrate is reduced by micro-organism in two ways. 1. Assimilatory Nitrate reduction. Assimilatory nitrate reduction mainly occurs during the anaerobic condition in absence of nitrogen. In this reduction process, nitrate and ammonia are used for the synthesis of organic … Read more

Catalase Biochemical Test

Catalase Biochemical Test Introduction Catalase is the name of an enzyme. This enzyme is nearly present in all living plants and animals which are always exposed to oxygen. Catalase enzyme is also present in aerobic and facultative anaerobic micro-organisms. Generally, this micro-organisms utilise oxygen and produce hydrogen peroxide and this hydrogen peroxide is toxic to … Read more

How is IMViC Biochemical test performed ?

How is the IMViC Biochemical test performed? In the IMViC test there are four biochemical tests and this test is carried out individually. IMViC test is carried out to identify members of the Enterobacteriaceae family. Each letter of IMViC test stand for individual test I – Indole production test. M – Methyl red test. V- … Read more

Carbohydrate Fermentation Test

Carbohydrate Fermentation Test Whenever a new micro-organism is discovered it becomes necessary to identify and catalogue it. For identification of this new microbe, it is first isolated and microscopically examined, and then further biochemically tested. Once these steps are carried out, we can identify this micro-organism. Biochemical tests are essential for the identification and classification … Read more

Microbial Culture Media Composition Part-3

Microbial Culture Media Composition Part-3 Here are some of the important culture media composition.   [table] 21. Eosin Methylene blue broth Peptone,- 1 gm Lactose,- 0.5 gm Eosin Y,- 0.04 gm K2HPO4,- 0.2 gm Methylene blue,-0.0065 gm Distill water,- 100 ml pH,- 7.2 [/table] Role a) Eosin Methylene blue broth is used for isolation of coliforms. … Read more

Microbial Culture Media Composition Part-2

Microbial Culture Media Composition Part-2 Please Watch the video given below Continued from Microbial Culture Media Composition Part-1 Some important media composition are given below. [table caption=”11. Hugh and Leifson’s medium”] Peptone,-0.2 gm NaCl,-0.5 gm K2HPO4,-0.03 gm Agar-agar,-0.3 gm Distill water,-100 ml 0.2 %  Bromothymol blue,-15 ml Glucose,-1 gm Paraffin oil,-10 to 20 ml pH,-7.1 … Read more

Microbial Culture Media Composition Part-1

Microbial Culture Media Composition Part-1 In nature, their are a variety of micro-organisms present and every single organism has some specific growth requirements like suitable temperature, pH, salt concentration nutrient requirements etc. If we want a desired culture of micro-organism we should provide them with the optimum and required growth conditions. On the basis of … Read more

Microbial Culture Media

Microbial Culture Media The micro-organism requires a base medium for their growth and it is called culture media. Nutrient media contains the substances that are used by organism synthesis of food, growth and survival. It fulfils all the nutritional requirements of the micro-organism. The nutrient media consist of water as the important ingredient plus organic … Read more

Staining of Fungus by using Lactophenol cotton blue

Staining of Fungus by using Lactophenol cotton blue Aim To stain fungal cells by using Lactophenol cotton blue. Approach Fungi are eukaryotic organism and they are classified into two main groups that is yeast and molds.Its cell wall is made up of chitin.Fungal cells have both macroscopic as well as microscopic structure. This microorganism is … Read more

Rickettsia Staining by using Gimenez Method

Rickettsia Staining by using Gimenez Method Aim To stain Rickettsia by using Gimenez staining method. Approach Rickettsia is a non-motile, Gram-negative,non-spore forming, pleomorphic bacteria. It is pleomorphic because it shows different shapes of bacteria that is cocci, rod and spiral shape. Rickettsia name was given by scientist Howard Ricketts. It is an obligate intracellular parasite … Read more