Capsule Staining Technique by Hiss method Introduction Capsule is the rigid, slimy and gummy covering of the bacterial cell which lies external to the cell wall. The cell that posses the capsule are called as capsulated cell and those who lack the capsule are called as the non-capsulated bacteria. Capsule is mainly made up of… Read More

Cell wall staining by Chances method Please watch the video given below Introduction Cell wall staining technique is a special staining technique in which we especially stain the cell wall of the bacterial cell. As we know cell wall is the outer most rigid covering of cell and depending upon the structure of cell wall… Read More

Acid Fast Staining Technique and its Details Introduction In nature, there is a variety of micro-organism each micro-organism have some special characters. Most of the microorganisms are easily stained by simple staining procedures. But there is some micro-organism that is not easily stained by this technique because they have a waxy covering on its surface.… Read More

Gram Staining Procedure and its Details Please watch the video given below Introduction Gram staining procedure was discovered by Han’s Christian Gram in 1884. Gram staining is a universal staining technique used for identification and classification of organisms. In this staining, method bacteria are classified into two groups that are-          … Read More

Negative Staining Method and its Mechanism Introduction Negative staining procedure is also called as Relief staining. Some bacteria have non-ionic charge or slime layer on their surface. Because of which the stain doesn’t penetrate inside the cell and the bacterial cells are not easily stained. In case of such bacterial cells negative method is used for… Read More

Simple Staining Procedure and its Mechanism Introduction Simple staining is a method of staining in which bacteria are stained by using a single stain. Simple staining is also called as monochrome staining or positive staining. Examples of simple stain are Methylene blue, Safranin, Malachite green, Basic fuchsin and crystal violet etc. In simple staining procedure… Read More

Stain and staining procedures Introduction Micro-organism can be identified by microscopic examination.These micro-organisms are very small, transparent, and invisible to our naked eyes so it is very difficult to observe these micro-organisms. These microbial cells are colourless so when these cells are suspended in the aqueous medium the refractive index of the cell and aqueous medium is… Read More