Spirochete Staining by Becker’s Method

Spirochete Staining by Becker’s Method

  • Spirochete are spiral shape bacteria .Becker’s staining is another special staining used to stain spirochete.
  • Other Spirochete staining techniques are Rue’s method and Levaditi’s method.

Aim

To stain spirochete by using Becker’s method.

Requirement

  1. Tartar sample.
  2. Fontana’s fixative.
  3. Fontana’s mordant.
  4. Becker’s stain.

Procedure

  1. First of all a sterile pin is used to take a sample of tartar from teeth.
  2. The tartar sample is mixed and homogenized in sterile distill water and further used as a suspension.
  3. A drop of suspension is taken on slide ,smear is prepared,air dried and  fixed by using chemical fixative that is Fontana’s fixative for 30 seconds for 2 times after interval of 1 minute.
  4. The slide is further washed with water,air dried and treated with Fontana’s mordant for 5 minutes.
  5. After 5 minutes the mordant is drained off and slide is given a water wash treatment.
  6. Further the slide is treated with Becker’s stain for 3 to 5 minutes.
  7. Finally slide is washed with water,air dried and observe under oil immersion.

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